The biggest feature in Biotech is the addition of families to RimWorld. With the expansion, players can now see their colonists have children and raise their own families. A number of options are available, including surrogate birth, and even the ability to gestate embryos in machines.

Along with the addition of families comes the addition of children to RimWorld. Players will be given plenty of tools to help raise children, keep them happy, and educate them by allowing them the ability to spend time in class, explore nature, or even chat on the radio, among other things.

Other features in Biotech include the addition of new xenohuman factions, the addition of the vampire-like Sanguophages, and the ability to turn colonists into a mechanitor—a person with brain implants that lets the psychically command semi-living machinnes called mechanoids. There are also new super-mechanoid bosses, as well as new pollution mechanics.