Nintendo has also confirmed price increases for software (physical and digital), Nintendo Switch Online memberships, and more.
Subscribers on Switch 2 will be able to use features with N64 titles, such as rewinding, changing controls, and applying a CRT filter.
Two other Game Boy titles are also available to subscribers of the service in the form of Survival Kids and The Sword of Hope.
Rare and Nintendo's acclaimed 1996 N64 fighting game is available now for all members of the service's costlier subscription tier.
Nintendo of America's Bill Trinen also considers the subscription service a “critical piece" of the console's experience.
Nintendo Switch 2's online library of GameCube titles will feature a CRT filter for even more of a throwback experience.
A leaker has claimed Nintendo will begin adding GameCube games to the service at some point within the Switch 2's launch year.
The racing title originally released for the N64 in 2000, before coming to the Nintendo DS in re-released form later in 2004.
Alongside the classic NES version of Tetris, its 1998 remaster from the Game Boy Color era is now playable on Nintendo Switch.
The acclaimed N64 platformer will be available to all Nintendo Switch Online + Expansion Pack subscribers later this month, Nintendo has announced.
"What excitement will the next year bring for Nintendo Switch Online + Expansion Pack members? Stay tuned to find out!"
The playtest will be available in the US, UK, France, Germany, Japan, Spain, and Italy. Registration opens on October 10th.
The service's subscribers also get access to three other SNES titles in Big Run, Kunio-kun no Dodgeball da yo Zen'in Shūgō!, and Cosma Gang the Puzzle.
The original NES Tetris is also coming to Nintendo Switch Online, while a new Maximum Cup event has also been announced for Tetris 99.
The original GBA roguelike dungeon crawler Pokemon Mystery Dungeon title will soon be available for Nintendo Switch Online + Expansion Pack members.
NSO subscribers can play Ubisoft and Nintendo's acclaimed turn-based tactics title at no additional cost for the next week.
Seven classic NES titles are available now for Nintendo Switch Online subscribers, including Nintendo's first ever fighting game.
Subscribers can experience Capcom's Game Boy Color versions today on Nintendo Switch, including the acclaimed Mega Man 3.
Three new SNES title have been added to the Nintendo Switch Online library with its latest update, Nintendo has revealed.
There's also a new Classic Min Prix, new Mirror Tracks for Knight League, a Recommended Challenges feature, and much more.